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Supplies Needed Plan based on on-demand use (up to 8 doses/month) or extended daily protocols

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Co-expression cell pellets were resuspended in 3 mL assay buffer (250 mM KCl, 2 mM MgCl 2 , 40 mM HEPES-KOH pH 7.9, 5 mM -mercaptoethanol, 10% (v/v) glycerol) and this was then divided into 1.5 mL for each of the different affinity pull-downs

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In summary, the relationship between BACH1 and o-quinones is a dynamic one, with BACH1 acting as a repressor and o-quinones contributing to its inactivation under OS conditions

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